Merck, 581322-U, Ascentis® C18 HPLC Column

Name: Ascentis® C18 HPLC Column
Brand: Merck
SKU: 581322-U

Size:1 ea, Ascentis RP18 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm

Catalog Number: 581322-U

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Category

Reversed-Phase HPLC Columns

Pack Size1 ea

TagsHPLC Columns Analytical Chemistry Analytical Chromatography

CollectionsMerck Analytical Chromatography Products & Lab Supplies Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.
Application

A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals: This paper details the application of Ascentis® C18 columns in a clinical setting to measure cortisol levels in urine. The method provides a fast, accurate, and reliable way to assess patient hormonal profiles, critical for diagnosing endocrine disorders (Zhao et al., 2022).

A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS: Utilizing Ascentis® C18, this study compares different analytical methods for quantifying ginsenosides. It showcases the column′s adaptability and precision in natural product research, essential for ensuring the quality and efficacy of herbal medicines (Hsu et al., 2022).

Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Highlighting the Ascentis® C18′s effectiveness in pharmaceutical formulations, this research demonstrates its capacity to analyze complex drug delivery systems, contributing to the development of more effective therapeutic solutions (Brugnera et al., 2022).

Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method: This publication illustrates the application of the Ascentis® C18 in veterinary medicine, providing crucial insights into drug stability and composition which are vital for regulatory compliance and animal safety (Padivitage et al., 2022).

Features and Benefits
Excellent retention
Symmetric peak shape
High reproducibility
Complete LC-MS compatibility

Other Notes
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Specifications

General

UNSPSC Code	41115700
eCl@ss	32110501
Web Name Suffix Override	L × I.D. 15 cm × 4.6 mm, HPLC Column
Web Name Override	Ascentis® C18 (3 µm) HPLC Columns
NACRES	SB.52
material	stainless steel column
agency	suitable for USP L1 (Similar to Phenomenex Luna C18)
product line	Ascentis®
feature	endcapped
manufacturer/tradename	Ascentis®
packaging	1 ea of
extent of labeling	25% Carbon loading
parameter	≤70 °C temp. range
technique(s)	HPLC: suitable
L × I.D.	15 cm × 4.6 mm
surface area	450 m2/g
surface coverage	3.7 μmol/m2
impurities	<5 ppm metals
matrix	fully porous particle
matrix active group	C18 (octadecyl) phase
particle size	3 μm
pore size	100 Å
operating pH range	2-8
application(s)	food and beverages
separation technique	reversed phase

FAQs

Question: What are the column dimensions and particle size?

Answer:The column is 15 cm × 4.6 mm and contains 3 µm particles.

Question: What pH and pressure range does the column support?

Answer:It supports pH 2–8 and pressures up to 400 bar (5801 psi).

Question: What stationary phase is used?

Answer:It uses an endcapped C18 phase on high-purity fully porous silica with 100 Å pores.

Application Note

Ascentis® C18 HPLC Column is designed for reversed-phase HPLC and LC-MS separations using an endcapped C18 bonded phase on high-purity silica. The column measures 15 cm × 4.6 mm and contains 3 µm spherical, fully porous silica particles with 100 Å pores. The stationary phase has 25% carbon loading, a surface area of 450 m²/g and surface coverage of 3.7 µmol/m². It is suitable for USP L1 methods and supports operation from pH 2–8, at temperatures up to 70 °C and pressures up to 400 bar (5801 psi). The combination of a conventional 4.6 mm analytical diameter and small 3 µm particles supports efficient small-molecule separations while retaining compatibility with standard HPLC hardware. The high-purity silica contains less than 5 ppm metals and the C18 chemistry provides hydrophobic retention for routine reversed-phase method development, quantitative analysis and LC-MS workflows.

Ordering

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