Merck, 581325-U, Ascentis®C18 HPLC Column

Name: Ascentis®C18 HPLC Column
Brand: Merck
SKU: 581325-U

Size:1 ea, Ascentis RP18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Catalog Number: 581325-U

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Category

Reversed-Phase HPLC Columns

Pack Size1 ea

TagsHPLC Columns Analytical Chemistry Analytical Chromatography

CollectionsMerck Analytical Chromatography Products & Lab Supplies Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.
Application

Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: This study utilized the Ascentis® C18 HPLC column for the separation of proteotypic tryptic peptides in the identification of insulin analogues, highlighting its application in targeted mass spectrometry for proteomic analysis (Qasem RJ et al., 2019).

Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben and propylparaben in a topical cream preparation.: This research developed and validated an HPLC method using the Ascentis® C18 HPLC column for the simultaneous assay of multiple compounds, demonstrating its robustness in stability testing of pharmaceutical formulations (Byrne J et al., 2014).

HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: The study employed the Ascentis® C18 HPLC column for metabolite profiling of propolis extracts using HPLC-DAD and HPLC-ESI-MS/MS, showcasing its effectiveness in complex natural product analysis (Pellati F et al., 2011).

Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: This article compared different HPLC column technologies, including the Ascentis® C18, highlighting its performance in fast separations critical for pharmaceutical process development (Abrahim A et al., 2010).

Features and Benefits
Excellent retention
Symmetric peak shape
High reproducibility
Complete LC-MS compatibility

Other Notes
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Specifications

General

UNSPSC Code	41115700
eCl@ss	32110501
Web Name Suffix Override	L × I.D. 25 cm × 4.6 mm, HPLC Column
Web Name Override	Ascentis® C18 (5 µm) HPLC Columns
NACRES	SB.52
material	stainless steel column
agency	suitable for USP L1 (Similar to Phenomenex Luna C18)
product line	Ascentis®
feature	endcapped
manufacturer/tradename	Ascentis®
packaging	1 ea of
extent of labeling	25% Carbon loading
parameter	≤70 °C temp. range
technique(s)	HPLC: suitable
L × I.D.	25 cm × 4.6 mm
surface area	450 m2/g
surface coverage	3.7 μmol/m2
impurities	<5 ppm metals
matrix	fully porous particle
matrix active group	C18 (octadecyl) phase
particle size	5 μm
pore size	100 Å
operating pH range	2-8
application(s)	food and beverages
separation technique	reversed phase

FAQs

Question: What are the dimensions of this Ascentis C18 column?

Answer:The column is 25 cm long with a 4.6 mm internal diameter.

Question: What particle and pore sizes are used?

Answer:It uses 5 µm fully porous silica particles with 100 Å pores.

Question: Which chromatographic mode is this column designed for?

Answer:It is an endcapped C18 reversed-phase HPLC column suitable for USP L1-type separations.

Application Note

Ascentis® C18 HPLC Column is used for reversed-phase HPLC separations of hydrophobic and moderately polar compounds in analytical workflows. This configuration uses a 25 cm × 4.6 mm stainless-steel column packed with 5 µm spherical, fully porous high-purity silica carrying an endcapped C18 stationary phase. The 100 Å pore structure and 25% carbon loading support strong reversed-phase retention, while the conventional 4.6 mm internal diameter fits widely used HPLC methods and instruments. The phase is suitable for USP L1 methods and can support routine pharmaceutical, chemical, food, and research separations that require a durable C18 column. Its 25 cm bed length provides useful resolving power for complex mixtures, especially where established methods depend on traditional 5 µm particles and standard-bore LC hardware.

Ordering

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