Merck, 58346-M, Tetracycline, Hydrochloride

General description
An antibiotic that inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit, preventing binding of aminoacyl tRNA to the A-site and blocking translocation. Used commonly for selection of the bacterial tetr gene whose product, a 399-amino acid membrane-associated protein, functions in active export of the drug from the cell.
Broad-based antibiotic agent that blocks protein synthesis by inhibiting binding of aminoacyl tRNA to the A-site of ribosomes. Induces cold shock-response and enhances P450 expression in bacteria.
Biochem/physiol Actions
Primary Target
binding of aminoacyl tRNA to the A-site of ribosomes
Preparation Note
Following reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for 1 month at -20°C. Agar plates are stable for 2 weeks at 4°C. Autoclaved media should be cooled to ≤55°C before adding tetracycline.
Other Notes
Kusano, K., et al. 1999. Arch. Biochem. Biophys. 367, 129.
Lech, K. and Brent, R. 1995. In Current Protocols in Molecular Biology, Vol. 1 (Ausubel, F.M., et al. eds.) John Wiley & Sons, New York Unit 1.4.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York, p. 1.6, A.6.
Chopra, I. and Howe, T.G.B. 1978. Microbiol. Rev. 42, 707.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)