Merck, 59140-U, SUPELCOSIL™ LC-ABZ HPLC Column

Name: SUPELCOSIL™ LC-ABZ HPLC Column
Brand: Merck
SKU: 59140-U

Size:1 ea, Amino-Benzene-Zone HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Catalog Number: 59140-U

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Category

Chiral HPLC Columns

Pack Size1 ea

TagsHPLC Columns Analytical Chemistry Analytical Chromatography

CollectionsMerck Analytical Chromatography Products & Lab Supplies Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
SUPELCOSIL LC-ABZ phase has a unique deactivation technology which provides excellent reversed-phase performance for basic compounds, as well as those that are acidic, polar neutral, and non-polar. Silanol suppressing practices such as using competing amines, operating at high ionic strength, and adjusting mobile phases to pH extremes are typically not required to obtain good peak shape on the LC-ABZ phase.
Application

Validation of a high-performance liquid chromatography method for the assay of and determination of related organic impurities in nevirapine drug substance.: This paper validates a method using the SUPELCOSIL™ LC-ABZ HPLC Column for assessing purity and impurities in HIV medication, showcasing the column′s high specificity and reproducibility (Li et al., 2000).

A comparative study of the reversed-phase HPLC retention behaviour of S-adenosyl-L-methionine and its related metabolites on Hypersil ODS and Supelcosil LC-ABZ stationary phases.: This comparative study provides insights into the retention behaviors of bioactive compounds on SUPELCOSIL™ LC-ABZ, affirming its versatility and robust performance in complex biochemical separations (Valkó et al., 1993).

Features and Benefits
• For acids, bases, zwitterions
• Increased polar retention relative to standard reversed-phases
• Unique selectivity
• Silanol deactivation
• Peak shape, efficiency, and retention are comparable to C8 phases for non-polar analytes
Other Notes
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Specifications

General

Quality Level
UNSPSC Code	41115700
eCl@ss	32110501
EC Number	231-545-4
Web Name Override	SUPELCOSIL™ LC-ABZ (5 µm) HPLC Columns
Web Name Suffix Override	L × I.D. 15 cm × 4.6 mm, HPLC Column
NACRES	SB.52
material	stainless steel column
agency	suitable for USP L60
product line	SUPELCOSIL™
feature	endcapped
manufacturer/tradename	SUPELCOSIL™
packaging	1 ea of
extent of labeling	12.0% carbon loading
parameter	≤70 °C temp. limit
technique(s)	HPLC: suitable
L × I.D.	15 cm × 4.6 mm
surface area	170 m2/g
surface coverage	surface coverage 3.4 μmol/m2
matrix	silica gel, spherical particle platform
matrix active group	amide, alkyl phase
particle size	5 μm
pore size	120 Å
pH range	2-7.5
application(s)	food and beverages
separation technique	reversed phase

FAQs

Question: What analyte types benefit from the LC-ABZ phase?

Answer:Basic compounds as well as acidic, polar neutral and nonpolar molecules.

Question: What packing dimensions and particle size are used?

Answer:150 × 4.6 mm with 5 µm particles and 120 Å pores.

Question: Is LC-ABZ the same stationary phase as LC-NH2?

Answer:No. LC-ABZ is a deactivated reversed-phase material; LC-NH2 is an amino-bonded phase.

Application Note

Supelco SUPELCOSIL LC-ABZ HPLC column uses a selectively deactivated silica-bonded phase designed for reversed-phase separation of basic, acidic, polar and nonpolar compounds. The 5 µm, 120 Å porous silica packing is contained in a 150 × 4.6 mm stainless-steel analytical column. Its alkyl-and-amide surface chemistry and suppressed residual silanol interactions support peak shape for basic analytes and provide different polar retention behavior from conventional C18 columns. This LC-ABZ phase is useful in method development where amine-containing or mixed-polarity compounds present retention and peak-symmetry challenges. The packing is endcapped and has a nominal 12% carbon loading. This is an LC-ABZ reversed-phase column, not the separate LC-NH2 amino column.

Ordering

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