Merck, 610205-50UG, Anti-Tie2 Mouse mAb (Ab33)

General description
Protein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
Recognizes the ~140-45 kDa Tie2 protein.
This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.
Immunogen
Human
a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag sequence
Application
Frozen Sections (see comments)
Immunoblotting (0.2-1 µg/ml, chemiluminescence)
Immunoprecipitation (see comments)
Paraffin Sections (not recommended)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
For frozen sections, tissues were treated with Triton™-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 8829.
Wong, A.L., et al. 1997. Circ. Res.81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer77, 51.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Disclaimer
Toxicity: Standard Handling (A)