Merck, 63289-U, BIOshell™ lgG 1000 Å C4, 2.7 μm HPLC Column

BIOshell™ lgG 1000 Å C4, 2.7 μm HPLC Column is designed for reversed-phase separation and characterization of immunoglobulin G and related intact or large protein species. The analytical column measures 150 mm × 2.1 mm and uses 2.7 µm superficially porous silica particles with an exceptionally wide 1000 Å pore structure. A bonded, endcapped C4 (butyl) phase provides hydrophobic reversed-phase selectivity while allowing large antibodies improved access to the particle surface. The superficially porous design combines a 1.7 µm solid core with an approximately 0.5 µm porous shell, shortening diffusion paths for macromolecules. Its specified molecular-weight range is 2,000–1,000,000 Da. The column supports pH 2–9, maximum temperature of 90 °C and pressures up to 1000 bar. Stainless-steel hardware encloses the packing, which has approximately 22 m²/g surface area and 0.6% carbon loading. The phase is compatible with HPLC, UHPLC and LC-MS workflows for antibody characterization and protein profiling. This product is a wide-pore C4 column classified as USP L26; it is not a conventional 100 Å C18 small-molecule column, and it is supplied as one 150 mm analytical column.