Merck, 646563-10X.5ML, DL-Dithiothreitol solution

General description
Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.

Application
DL-Dithiothreitol solution has been used:
in western blot analysis
as a supplement in lysis buffer to study its effects on sperm lysis
as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds

Features and Benefits
Low toxicity and reduced odor compared to other thiol compounds.