Merck, 648313, TRIS Hydrochloride

General description
Absorbance (1.0 M, H2O, 260 nm):≤0.1.
Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
Widely used as a biological buffer (absorbance: 2O). A 1.0 M solution in H2O has a pH range of 3.5-5.0 and a pKa of 8.3 at 20°C.
Application

Effect of Organic Solvents on the Activity, Stability and Secondary Structure of asclepain cI, Using FTIR and Molecular Dynamics Simulations.: This study uses Tris hydrochloride as a buffer to assess the stability and structural changes in the asclepain cI enzyme, providing insights into enzyme applications in industrial and medical biotechnology (Origone et al., 2024).

Microencapsulation of Omeprazole by Lactobacillus acidophilus ATCC 4356 Surface Layer Protein and Evaluation of its Stability in Acidic Condition.: Research demonstrates the use of Tris hydrochloride buffer in stabilizing microencapsulated omeprazole, aimed at enhancing drug delivery in acidic environments (Mobarak Qamsari et al., 2020).

Other Notes
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)