Merck, 648465-10GM, Triton™ X-100 Detergent, Hydrogenated

General description
Nonionic detergent used for the solubilization of membrane-bound proteins. Lacks the UV absorbance and fluorescence of TRITON™ X-100 (Cat. No. 648462), which causes interference in spectophotometric monitoring and assays. Has a critical micelle concentration (CMC) of 250 µM.
Application

The utilization of Triton X-100 for enhanced two-dimensional liquid-phase proteomics.: This study explores the role of Triton X-100 in enhancing protein separation techniques in biochemistry, particularly emphasizing its application in two-dimensional liquid-phase proteomics to improve resolution and reproducibility (Kim et al., 2011).

Effects of environment on the photophysical characteristics of mesotetrakis methylpyridiniumyl porphyrin (TMPyP).: Research focuses on the influence of different solvent environments, including those containing Triton X-100, on the photophysical properties of porphyrins, highlighting its utility in enhancing solubility and stability in biochemical assays (Gonccedil;alves et al., 2011).

Processing of tissue culture cells.: The work describes methods for processing tissue culture cells where Triton X-100 is used to permeabilize cells for various staining procedures, crucial for molecular biology and cellular biochemistry studies (Bratthauer, 2010).

Packaging
Packaged under inert gas
Preparation Note
Following dilution in H₂O, store at room temperature. Diluted stock solutions are stable for up to 3 months at room temperature.
Other Notes
Fernandez, J., et al. 1992. Anal. Biochem.201, 255.
Tiller, G.E., et al. 1984. Anal. Biochem.141, 262.
Ashani, Y. and Cantravas, G.N. 1980. Anal. Biochem.109, 55.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Disclaimer
Toxicity: Harmful (C)