Merck, 67432-1VL, Campylobacter Millichrome™ plus Supplement

General description
Campylobacter Millichrome™ plus supplement is a selective media supplement for the detection of thermotolerant Campylobacter. The supplement provides additional selectivity to the Campylobacter Millichrome™ plus Agar Base. Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 1 vial of supplement sufficient for 5 liters of final media. The supplement must be added to the melted Campylobacter Millichrome™ plus Agar Base at 45-50 °C.
Application
Campylobacter Millichrome™ plus supplement is a selective chromogenic culture media supplement intended for use in the qualitative direct detection, differentiation and presumptive identification of pathogenic biotypes of thermotolerant Campylobacter from various samples
Preparation Note
In a transparent vessel, add 210 mg of Campylobacter Millichrome™ plus Supplement to 10 ml purified water.
Swirl well until complete dissolution.
Filter to sterilize at 0.45 μm.
Add the 10 ml of the supplement solution to 1 liter of the melted Campylobacter Chromocult® plus Agar base at 45-50 °C.
Swirl or stir gently to homogenize.

Analysis Note
The Campylobacter colonies are colored red after 48 h of incubation at an optimal growth temperature of 42°C, whereas the growth of other species is inhibited or forms blue colonies after incubation at the same temperature.
Other Notes
Composition in g/l:
(1 vial is sufficient for 5 L of final media)
Chromogenic and selective mix 1.05 g
Legal Information
CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany