Merck, 68075-1VL, C. perfringens Millichrome™ plus Supplement 2

General description
C. perfringens Millichrome™ plus Supplement 2 is a selective media supplement for the detection and enumeration of C. perfringens. Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. One vial (0.6 g) of the supplement is sufficient for 5L of final media. The prepared supplement is added to the melted C. perfringens Millichrome™ plus Agar Base at 45-50 °C.
Application
C. perfringens Millichrome™ plus Supplement 2 is a selective chromogenic media supplement intended for use in the qualitative detection and enumeration of C. perfringens from various samples.
Packaging
1 vial à 0.6g (with screw cap) is sufficient for 5L
Preparation Note
Add 0.12 g of C. perfringens Millichrome™ plus supplement 2 in 1 mL of purified water.
Filter to sterilize at 0.45 μm
Add 1 mL/L of the supplement 2 solution to the melted base at 45/50 °C.

Analysis Note
The Clostridium perfringens is detected by a characteristic orange color after 24 h at an optimal growth temperature of 37°C, while Clostridium difficile forms blue to green colonies after incubation at the same temperature.
Legal Information
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany