Merck, 684274, β1,2-Xylosidase, <i>Xanthomonas</i> sp.

General description
Native β1,2-Xylosidase from Xanthomonas species. Catalyzes the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of α1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.
Note: 1 mU = 1 milliunit.
Physical form
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Preparation Note
Following initial use, refrigerate (4°C).
Other Notes
One unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.
Reese, A.J. and Doening, T.L. 2003. Mol. Microbiol.50, 1401.
Legal Information
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Disclaimer
Toxicity: Standard Handling (A)