Description
General description
HIS-BIND® purification kit includes pre-tested buffers specifically designed for IDA-based HIS-BIND Resin, enabling convenient and rapid one-step purification of proteins by metal affinity chromatography. It uses the HIS TAG®/HIS-BIND® technology, that leverages the affinity between neighboring histidine in the HIS TAG sequence and immobilized metal ions (such as Ni²⁺ or Co²⁺).
Application
HIS-BIND® Purification Kit has been used for: the purification of N-terminal 6-histidine-tagged aldehyde dehydrogenase isozyme, ALDH1A1 under nondenaturing conditions by nickel-NTA affinity chromatography, the purification of virus binding protein specific for Poliovirus Type 1, the purification of recombinant oE7, huhsp70 and oE7/huhsp70 proteins, to study the effective antitumor efficacy of human hsp70 and HPV16 oE7 fusion protein vaccine, the purification of recombinant exFPR1, MTBHsp70 and MTBHsp70-exFPR1 fusion proteins to study their role in cervical cancer immunotherapy
Features and Benefits
Includes HIS-BIND® resin, HIS-BIND® buffer kit and chromatography columns.
Solutions are included for Ni²⁺ charging, binding, washing, and elution.
Columns are pre-packed with 1.25 ml bed volume of Ni²⁺-charged HIS-BIND resin.
Other Notes
•10 mlHis•Bind Resin
•1His•Bind Buffer Kit
•pkg/4Chromatography Columns
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
