Merck, 70850-3, FastPlax Titer Kit - Novagen

General description
The FastPlax Titer Kit is designed for determination of baculovirus titers in just 24 to 48 hours instead of three to four days as in other methods. Classical determination of baculovirus titer has relied on the appearance of plaques, but the FastPlax Kit takes advantage of the appearance of detectable levels of AcNPV gp64 glycoprotein on the cell surface as early as 8 to 24 hours postinfection. Detection is based on a high affinity monoclonal antibody against gp64, which is added directly to fixed cells. Antibody binding is detected with Goat Anti-Mouse IgG β-Galactosidase Conjugate followed by enhanced color development with X-Gal/NBT substrates. Plaques are clearly distinguished by their dark blue color. The kit contains sufficient reagents to perform five 6-well plate assays.
Packaging
Plastic ampoule
Preparation Note
Multiple storage requirements
Other Notes
2 × 50 ml- 10X TBST (100 mM Tris-HCl, pH 8.0, 1.5 M NaCl, 1% Tween® 20)
6 ml- 10% Gelatin (contains 0.02% azide)
10 µl- FastPlax Antibody
300 µl- Goat-Anti-Mouse β-Galactosidase Conjugate
300 µl- NBT (83 mg/ml in 70% dimethylformamide)
300 µl- X-gal (50 mg/ml in dimethylformamide)

Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS