Description
General description
The Assurance® GDS Shiga Toxin Genes (O157) Tq is an automated nucleic acid amplification system for the detection of Shiga Toxin producing E. coli O157:H7. The Shiga Toxin Genes (O157) assay is designed for use in conjunction with Assurance® GDS E. coli O157:H7 Tq assay to detect shiga toxin genes (stx1 and stx2) present in presumptive positive E. coli O157:H7 samples.
Application
Assurance® GDS Shiga Toxin Genes (O157) Tq is suitable for the detection of Shiga Toxin producing E. coli O157:H7 in foods, ingredients, and environmental samples.
PCR, Immunomagnetic Separation (IMS)
Features and Benefits
Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 6 or Top 7 O groups, samples are analyzed for the presence of eae with the GDS Top 6 or 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance® GDS Shiga Toxin Genes (Top 6 or Top 7) assay
Confirm Assurance® GDS E. coli O157:H7 positives samples with Assurance® GDS Shiga Toxin Genes (O157) assay
Single-step 10-18 h enrichment in mEHEC; enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC
Other Notes
Shiga Toxin Genes Amplification Tubes Tq
O157 Concentration Reagent
Resuspension Buffer Tq
Wash Solution
Legal Information
AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of Merck KGaA, Darmstadt, Germany
Assurance GDS is a registered trademark of BioControl Systems, Inc.