Description
General description
The pETcoco System combines the advantages of T7 promoter-driven protein expression with the ability to control plasmid copy number (Sektas 2003). In the presence of 0.2% glucose, pETcoco recombinant plasmids are maintained at one copy per cell. In this single-copy state, pETcoco clones are extremely stable because background expression and consequent gene rearrangements or deletions are virtually absent. For plasmid preparation, the copy number can be amplified to approximately 40 copies per cell independently of target gene expression by addition of L-arabinose to the culture medium. Both target gene expression and plasmid plus insert amplification are induced upon addition of IPTG. Both pETcoco-1 and pETcoco-2 vectors feature an N-terminal His•Tag and internal S•Tag sequences, both of which are cleavable by enterokinase, and an optional C-terminal HSV•Tag sequence. NovaF- Competent Cells for cloning target genes and Tuner(DE3) Competent Cells for expression are supplied with the pETcoco-1 and pETcoco-2 Systems.
Coexpression
The pETcoco-1 plasmid contains a pET vector-compatible origin of replication and chloramphenicol resistance marker, making it suitable for transformation into cells already containing ampicillin- or kanamycin-resistant pET or pETDuet-1 recombinants to enable the coexpression of multiple target genes in the same cell. The ampicillin-resistant pETcoco-2 plasmid is compatible with pET host strains supplying rare codon tRNAs and/or T7 lysozyme on a chloramphenicol-resistant plasmid, and with kanamycin-resistant pET recombinants for coexpression purposes. pETcoco-2 may also be used for coexpression with pACYCDuet-1 recombinants with appropriate host strains.
Other Notes
Sektas, M. and Szybalski, W. 2003. inNovations 14, 6.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Clickhere for Enduser Declaration (EUD) Form
Toxicity: Multiple Toxicity Values, refer to MSDS
