Merck, 72485-100MG, Nile Red
Size:100 mg, Nile Blue A Oxazone, suitable for microscopy
Available Catalog Numbers
Catalog Number: 72485-100MG
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Category
Pack Size100 mg
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| UNSPSC Code | 12171500 |
|---|---|
| NACRES | NA.32 |
| PubChem Substance ID | 57652419 |
| EC Number | 230-966-0 |
| Beilstein/REAXYS Number | 279110 |
| MDL number | MFCD00011639 |
| InChI key | VOFUROIFQGPCGE-UHFFFAOYSA-N |
| InChI | 1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3 |
| SMILES string | CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34 |
| form | crystals |
| Quality Level | 100 |
| mol wt | 318.37 g/mol |
| color | brown to very dark brown |
| mp | 203-205 °C (lit.) |
| solubility | methanol: 0.01 g/10 mL (red to very dark red) |
| λ<sub>max</sub> | 553 nm |
| suitability | suitable for microscopy |
Answer:Nile Red is used in fluorescence microscopy to stain intracellular lipid droplets and other hydrophobic lipid-rich structures.
Answer:It is a lipophilic phenoxazone dye with formula C20H18N2O2 and molecular weight about 318.37 g/mol.
Answer:The research reagent is supplied as 100 mg of solid Nile Red dye.
Nile Red is a lipophilic phenoxazone fluorescent dye widely used in microscopy to visualize lipid-rich structures, especially intracellular neutral lipid droplets. Its fluorescence response depends strongly on the polarity of its immediate environment, making it useful for identifying hydrophobic compartments in cells and biological samples. This 100 mg research-grade solid has a molecular formula of C20H18N2O2 and a molecular weight of approximately 318.37 g/mol. It is suitable for preparing staining solutions for fluorescence microscopy and lipid-localization experiments. Dye concentration, solvent, excitation and emission settings, sample fixation and washing conditions require optimization for the specimen. Nile Red is a staining reagent, not an antibody or a quantitative lipid assay kit with built-in standards.
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