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| Source | Mouse Frizzled-10, amino acids Ile22-Phe166 (Accession # NM_175284.3) with a C-terminal human IgG1 (Pro100-Lys330) Fc tag was expressed in CHO cells. |
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| Molecular Mass | The 384 amino acid recombinant protein has a predicted molecular mass of approximately 43.3 kD. The DTT-reduced protein migrates at approximately 55 kD and and non-reduced protein migrates at approximately 110 kD by SDS-PAGE. The predicted N-terminal amino acid is Ile. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2 |
| Endotoxin Level | Less than 0.1 EU per µg protein as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | ED50= 0.5 - 2.0 µg/mL as measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells induced by 50 ng/mL of recombinant Wnt3a (Cat. No. 772301). |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Homodimer |
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| Distribution | Expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney. |
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| Function | Receptor for Wnt proteins that functions in the canonical Wnt/beta-catenin signaling pathway. |
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| Ligand/Receptor | Wnt7a, Wnt7b, Wnt1, Wnt3a |
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| Bioactivity | Measured by its ability to inhibit Wnt3a activity in Topflash assay. |
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| Biology Area | Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure |
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| Molecular Family | Soluble Receptors |
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| Antigen References | Koike J, et al. 1999. Biochem Biophys Res Commun. 262:39-43 Kawakami Y, et al. 2000. Mech Dev. 91:375-378 Dann CE, et al. 2001. Nature. 412:86-90 Nagayama S, et al. 2005. Oncogene. 24:6201-6212 Kwon HS, et al. 2009. Mol Cell Biol. 29:2139-2154 Galli LM, et al. 2014. Dev Dyn. 243:833-843 |
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| Gene ID | 93897 |
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| UniProt | View information about Frizzled-10 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | FZD10, CD350, Fz-10 |
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