Merck, 803391-100MG, SM(PEG)24 (PEGylated, long-chain SMCC crosslinker)

General description
SM(PEG)n NHS- and maleimide-activated PEG compounds for crosslinking between primary amines (NH2) and sulfhydryl (SH) groups in proteins and other molecules. A complete set of compounds are offered, each having the same heterobifunctional structure (NHS-PEGn-Maleimide) but differing in the number of discrete ethylene glycol units ( n = 2, 4, 6, 8, 12 or 24). The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The maleimide group reacts with reduced sulfhydryls at pH 6.5-7.5 to form stable thioether bonds
Application
SM(PEG)24 can be used as a crosslinker in:
The study of chemical cross-linking of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and RNAses using the high-mass MALDI-MS technique.
Preparation of protein G-liposomal nanovesicles applicable as sensors in the detection of gentamycin in the milk sample.
Conjugation of catalase to streptavidin, which is helpful in the detection of analytes at very low concentrations using ELISA technique.

Features and Benefits
Reactive groups: NHS ester and maleimide
Reactive toward: amino and sulfhydryl groups
Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
Polyethylene glycol spacer arms help maintain conjugate solubility
Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
Ideal for performing controlled, two-step protein conjugations (see Sulfo-SMCC)

Disclaimer
This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.