Merck, 83264-500ML-F, HEPES buffer solution

General description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application
HEPES has been used:
As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
As a component of dissection buffer for culturing rat cortical neurons
Along with MgCl₂, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
As a component of Danieu′s aquarium water used in agarose gel preparation