Merck, 85011422-DNA-5UG, BSC1

Biochem/physiol Actions
Continuous cell line which when infected with SV40 virus develops characteristic cytopathic effect.
Monkey African green kidney epithelial
Preparation Note
EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 10% FBS / FCS (F2442).
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin trypsin/EDTA; 5% CO₂; 37 °C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].