Merck, 85042305-1VL, B65

Application
Neurobiological studies
Biochem/physiol Actions
Ethyl nitrosourea induced tumour cell line. Neuronal cells are useful for research into the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Cell morphology significantly affected when grown in serum free media.
Rat nervous tissue neuronal
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].