Merck, 85062501-DNA-5UG, 53B3

Immunogen
Sheep red blood cells
Application
Fusion partner
Biochem/physiol Actions
Mouse x Bovine 53B3 x Jersey calf lymph node
This HAT sensitive line can be used as a fusion partner in the production of heterohybridomas.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].