Merck, 85062504-1VL, 111A3

Immunogen
Sheep red blood cells
Application
This line has been used as a HAT sensitive fusion partner in the production of heterohybridomas
Biochem/physiol Actions
Mouse 53B3 x Bovine Jersey Calf lymph node
Non Ig secreting
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS)
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].