Merck, 85082302-DNA-5UG, BW5147.G.1.4.OUA/R.1

General description
For complete product information, please see BW5147.G.1.4.OUA/R.1
Application
Fusion partner
Biochem/physiol Actions
Mouse AKR/J T cell lymphoma
This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.
Preparation Note
Maintain cultures between 1-6x100,000 cells/ml; 5% CO₂; 37°C.
SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].