Merck, 8510258100, 5-Carboxyfluorescein

General description
This high purity fluorescent dye (λex494 nm, λem 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment [¹].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

[¹] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.
Application
5-Carboxyfluorescein Novabiochem®. CAS 76823-03-5, molar mass 376.32 g/mol.
Analysis Note
Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem® products please click here.
Other Notes
Replaces: 01-63-0112
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany