Merck, 8510728025, 6-Carboxyfluorescein

General description
This high purity fluorescent dye (λem494 nm, λex518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or protected by tritylation prior to acylation or hydrazine treatment[¹].

Literature references

[¹]R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.
Analysis Note
Color (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 95 %
Assay (HPLC, area%): ≥ 98.0 % (a/a)

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Other Notes
Replaces: 01-63-0113
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany