Merck, 857461P-1MG, 16:1 aldehyde-d5

General description
Fatty aldehydes are enzymatically converted to fatty alcohols and fatty acids. When assessing S1P lyase enzymatic activity, the estimation of fatty aldehyde stability during the reaction is required. The (2E)-hexadecenal(d5) (857461) is suitable as the internal standard for LC/MS/MS approaches to quantify the product of S1P degradation by S1P lyase, (2E)-hexadecenal; however, a natural (2E)-hexadecenal (857469) is needed to evaluate its stability during enzymatic reaction. The method describing the details of the use of both (2E)-hexadecenal(d5) and (2E)-hexadecenal for LC/MS/MS-based characterization of S1P lyase reaction is in preparation for publication by Berdyshev et al.Personal communication from Evgeny Berdyshev, Ph.D., Assistant Professor, University of Illinois at Chicago.
Application
16:1 aldehyde-d5 has been used as an internal standard in lipid extraction from lung tissue and liquid chromatography/with tandem mass spectrometry analysis (LC/MS/MS). It has also been used as a standard in lipid extraction from plasma, derivatization of 2E-hexadecenal for quantification in quadrupole/time-of-flight (QTOF) mass spectrometer connected with a rapid-resolution-liquid-chromatography (RRLC).
Biochem/physiol Actions
16:1 aldehyde is a predominant fatty aldehyde in phospholipid of bovine adipose tissue, Lactobacillus and few bacteria like Selenomonas ruminantium, when grown on glucose and even-numbered volatile fatty acids. It is one of the main aldehydes in plasmalogen of notched brittle star, Ophiura sarsi.
Packaging
5 mL Amber Glass Screw Cap Vial (857461P-1mg)
5 mL Amber Glass Screw Cap Vial (857461P-5mg)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC