Merck, 86030310-1VL, AFRC MAC 57

Immunogen
Sonicated bacteroid material from Rhizobium leguminosarum strain 3841 and strain B155
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat LOU/Iap spleen
The antibody is directed against lipopolysaccharide components of the bacterial cell wall and also with lipopolysaccharide from free-living cultures of Rhizobium strains 3841 and B155.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].