Merck, 86030311-1VL, AFRC MAC 63

Immunogen
Peribacteroid material followed by peribacteroid material depleted of bacterial lipopolysaccharide
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat DA spleen
The antibody is directed against peribacteroid material from Rhizobium-induced root nodules of Pisum sativum. The antigen is also present on plasma membranes and in the Golgi bodies, but not in endoplasmic reticulum (ER). The antibody also reacts with peribacteroid membranes from nodules of Vicia and lupin.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].