Merck, 86062401-1VL, BL-3

Application
Virus studies
Biochem/physiol Actions
BL-3 was established from a neoplastic lymph node of a 3 month old male calf. BVDV positive.
Bovine leukaemic lymphoblast
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Maintain cultures between 5-9x100,000 cells/ml; 5% CO₂; 37°C. Cells should not be over diluted.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].