Merck, 86093001-DNA-5UG, BC3H1

Application
Acetylcholine receptor studies.
Biochem/physiol Actions
Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.
Mouse brain tumour
Preparation Note
DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].