Merck, 86110401-DNA-5UG, 3T3 clone A31 Cell Line from mouse

Application
Tumourigenicity and virus studies: SV40 and MSV
3T3 clone A31 cell line from mouse has been used:
to study in vitro cytotoxicity and genotoxicity of retinyl palmitate–loaded poly(lactide-co-glycolide) nanoparticles for the topical treatment of skin diseases
for the characterization of the phototoxicity of Vemurafenib, which is a B-Raf serine/threonine kinase inhibitor
for the phototoxicity assessment of systemically applied compounds and drug candidates

Biochem/physiol Actions
Cells have now lost their contact inhibition.
Mouse BALB/c embryo
Preparation Note
DMEM + 2mM Glutamine + 5% New Born Calf Serum (NBCS) + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].