Merck, 86112690-1VL, AFRC MAC 74

Immunogen
Nitrate reductase purified from the leaves of 6-8 week old spinach plants - Spinacea oleraceal
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat F344 spleen
The antibody is specific for NADH nitrite reductase and inhibits reduction of nitrate to nitrate using NADH electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) or methyl viologen. The antibody is directed against NADH-NR if mono and dicotyledons.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].