Merck, 87030901-DNA-5UG, 745C2

Application
Haematopoiesis studies
Biochem/physiol Actions
Mouse erythroleukaemic lymphoblast
This cell line is a clone isolated from the parent line 745 TG-12. The cells are resistant to oubain and thioguanine and deficient in HGPRT. Haemoglobulin synthesis can be induced with 1.5% DMSO or 1mM oubain.
Preparation Note
Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].