Merck, 87081901-DNA-5UG, 31F4L

Biochem/physiol Actions
31F4L has been transfected with Epstein Barr virus DNA but is not considered to be transformed, i.e., does not form foci on agar and does retain epithelial morphology.
Monkey African Green kidney
Preparation Note
DMEM (D6546) + 2 mM L-Glutamine (G7513) + 5-10% FBS / FCS (F2442).
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 °C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].