Merck, 87091801-DNA-5UG, ATC-10 (Aedes aegypti)

Application
Virus studies: Arboviruses
Biochem/physiol Actions
Derived from pooled newly hatched Aedes aegypti larvae. The cells are susceptible to a range of arboviruses.
Mosquito larvae
Preparation Note
Mitsuhashi and Maramorosch Insect Medium (pH 7.0) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5 x10,000 cells/cm² using 0.25% trypsin for 5 mins only 28°C. After quenching the trypsin with fresh mediumdislodge the cells by gentle scraping.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].