Merck, 88022404-1VL, AFRC IAH-CC14

Immunogen
Bovine thymocytes
Biochem/physiol Actions
Mouse NS1 x Mouse BALB/c spleen
The antibody is directed against a thymic differentiation antigen in cattle. The antigen is expressed on 50-60% of bovine thymocytes, but not on peripheral blood mononuclear cells. The antigen recognised is the bovine equivalent of CD1.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].