Merck, 88031149-DNA-5UG, BAE-1

Biochem/physiol Actions
Derived from the aorta of a Friesian cow. Cells form "sprouts" after confluence. Infected with Bovine Viral Diarrhoea Virus (BVDV) which is a common contaminant of ruminants and is non-hazardous to humans. Secretes proteoglycans and collagen.
Bovine aortic endothelium
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. There is a tendancy for the cells to grow fibroblast-like until they are confluent. Once confluent the endothelial morphology prevail
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].