Merck, 88100507-1VL, BW5147.G.1.4.OUAR.1

Application
Resistance and hybridisation studies
Biochem/physiol Actions
Mouse AKR/J T cell lymphoma
This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100βM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.
Preparation Note
DMEM + 2mM Glutamine + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].