Merck, 88111402-1VL, 7D4

Immunogen
Murine IL-2 dependent HT-2 cells
Application
The isotype of the monoclonal antibody produced by this cell line is rat IgM, kappa.
Biochem/physiol Actions
Mouse Sp2/0 x Rat Lewis spleen
The antibody is directed against the murine IL-2 receptor ligand complex present on activated murine T and B cells. It inhibits proliferation of activated cells in the presence of IL-2, but does not block the binding of IL-2.
Preparation Note
DMEM + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].