Merck, 88121201-DNA-5UG, ARH 77

Biochem/physiol Actions
Derived from a 33 year old female with plasma cell leukaemia. The cells synthesise IgG1 (kappa-light chain) and are EBNA positive.Although the cells are reported to originate from a patient with a plasma cell leukaemia , it has now been shown that this is in fact an EBV transformed B lymphoblastoid cell line .
Human plasma cell leukaemia
STR-PCR Data: Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8,9.3
TPOX: 8
vWA: 17
Preparation Note
Maintain cultures between 1-4x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].