Merck, 89030316-DNA-5UG, A2

Application
Enzymatic studies
Biochem/physiol Actions
Hamster Chinese ovary
Overproduces ornithine decarboxylase in response to polyamine deprivation.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + Ham′s F12 (without Putrescine) (1:1) + 0.1% BSA.
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 minutes or until cells detach. Culture cells at 37°C; 5% CO₂. Pre-soak flasks in porcine
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].