Merck, 89031603-DNA-5UG, BT

Biochem/physiol Actions
Bovine turbinate, BVDV negative
Derived from new-born bovine turbinate tissue. Used in propagation of BVDV and IBRV, some strains of parainfluenza 3 virus (P1-3V), some types of bovine adenovirus and enterovirus. Negative for BVDV.
Preparation Note
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Horse Serum (HS)
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂;37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].