Merck, 89071903-DNA-5UG, AK-D

Application
Virus studies: panleucopenia, feline herpes and feline picornaviruses
Biochem/physiol Actions
A clonal derivative of mass cultures of foetal feline lung after 14 passages in vitro. Susceptible to panleukopenia, feline herpes and feline picornaviruses.
Foetal cat lung normal
Preparation Note
Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin and 0.03% EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].