Merck, 89072613-DNA-5UG, A6

Application
Replication studies
Biochem/physiol Actions
Derived from normal kidneys of an adult male toad (Xenopus laevis). A6 cells support the replication of Granoff′s frog viruses but not the Lucke inclusion-tumour associated virus.
South African clawed toad kidney
Preparation Note
NCTC 109 + 15% Distilled H₂O + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 27-28°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].