Merck, 89120558-1VL, AFRC MAC 257

Immunogen
Purified auxin binding protein
Application
The isotype of the monoclonal antibody produced by this cell line is rat IgM.
Biochem/physiol Actions
Mouse NS0/U x Rat F344 spleen
The antibody recognises the 22kDa major auxin binding protein polypeptide and. In addition a minor 21kDa species is recognised.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].