Merck, 89120566-DNA-5UG, AGLCL

Application
Heterozygosity studies
Biochem/physiol Actions
Human Caucasian normal B cell
Normal B cell line of a 72 year old Caucasian male. From the same patient as LUDLU 1 (Sigma Catalogue number. 92012463).
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 12
D16S539: 13,14
D5S818: 11,12
D7S820: 10,12
THO1: 6,9.3
TPOX: 8,11
vWA: 17,19
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells grow as large cell aggregates; resuscitate in 8ml media with flask standing up until cells start to proliferate well.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].