Merck, 90011807-DNA-5UG, 149BR

Application
DNA repair studies
Biochem/physiol Actions
Human skin fibroblast
Initiated on 6th November 1987 from a biopsy of a 51 year old normal male. The cells have been used in radiation survival studies. EBV-transformed lymphocytes designated LB182 (Sigma Catalogue number. 98062317) have been isolated from the same individual. This has been confirmed by STR profiling at ECACC.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].