Merck, 90011887-DNA-5UG, 1306

Application
Transfection studies
Biochem/physiol Actions
A HPRT- derivative of the SV40 transformed human fibroblast line GM0637. The cells were selected in 5μg/ml thioguanine and are useful for transfection experiments.
Human skin fibroblast
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 13
D16S539: 12,13
D5S818: 11
D7S820: 10
THO1: 7,9
TPOX: 9,11
vWA: 16,17
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].